Recombinant Alkaline Phosphatase anticorps (CF®740)

N° du produit ABIN7649375

L’anticorps Lapin Monoclonal anti-Alkaline Phosphatase a été validé pour WB, IHC et IHC (fp). Il convient pour détecter Alkaline Phosphatase dans des échantillons de Humain.

Aperçu rapide pour Recombinant Alkaline Phosphatase anticorps (CF®740) (ABIN7649375)

Antigène: Alkaline Phosphatase (ALP)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Alkaline Phosphatase est conjugé à/à la CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: ALPP-2899R

Détail du produit anti-Alkaline Phosphatase anticorps (CF®740)

Fonction: Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) (ALPP/2899R), CF740 conjugate

Attributs du produit: This antibody reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP This antibody reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human ALPP protein

Isotype: IgG

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.25-0.5 μg/mL for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: HepG2 cells. Placenta or seminoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Alkaline Phosphatase

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase

Sujet:

Synonyms: Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, ALP, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP-1, PLAP1

Gene Symbol: ALPP

Tissue Expression: Placenta

Poids moléculaire: 70 kDa

ID gène: 250

UniProt: P05187